Induction of Somatic Embryogenesis Moringa oleifera Lam.With Addition of Various Concentration Naphtalene Acetic Acid (NAA) and Kinetin in Medium MS in-vitro

Abstract

Moringa (Moringa oleifera) is the plants which have high nutritional content of minerals and vitamins. In Indonesia, the cultivation of Moringa still use vegetative propagation. Therefore, the resulting seedlings are limited and have a low quality of seeds. To solve the problems, rapid Moringa propagation and sustainable high quality of seeds through in-vitro culture are needed. This study aimed to determine the effect of somatic embryogenesis induction of Moringa leaves due to the adding variation of plant growth regulators concentration of Napthalene Acetic Acid (NAA) and Kinetin in MS medium in-vitro. This study was conducted using a completely randomized design with four treatments, are M1 (MS 0), M2 (MS + NAA 0.5 ppm + Kinetin 1 ppm), M3 (MS + NAA 1 ppm + Kinetin 1 ppm), and M4 (MS + NAA 1 ppm + Kinetin 0.5 ppm) and there were three replications of each treatments. Parameters observed that were the rate of induction time, color and texture of callus. Data were analyzed using analysis of descriptive. The results showed that the addition of plant growth regulators NAA and Kinetin at various concentration affected callus growth of Moringa leaves on MS medium in-vitro. The medium concentration of M2 (MS + NAA 0.5 ppm + Kinetin 1 ppm) was the optimal concentration on callus induction (on fourteenth day), the callus has white color and compact texture.

Keywords—Kinetin, Moringa leaf, Moringa oleifera, Napthalene Acetic Acid (NAA), somatic embryogenesis