Production and Purification of Lipase Enzyme from Bacillus licheniformis F11.4 as Biodetergent

Ramadhan Iskandar, Muhamad Sahlan, Siswa Setyahadi

Abstract

Lipase enzyme can use in many industries, such as food, detergent, textile, pharmaceutical, biodiesel and agrochemical. In this research, lipase enzyme tested its ability as an additive in detergent. Lipase enzyme produced by Bacillus licheniformis F11.4 purified two times, by stirred-cell ultrafiltration and dialysis. Purification with stirred-cell ultrafiltration conduct at optimum operation condition; 50 kDa membrane size, 30 psi nitrogen gas pressure, and maximal concentrate. Washing efficiency of purified lipase enzyme on cloth with artificial dirt, olive oil, is 16,07%, if combine with detergent is 34,88%, and can enhance washing efficiency by detergent up to 6,05%.

Keywords— Bacillus licheniformis F11.4; dialysis; lipase enzyme; stirred-cell ultrafiltration

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