Potention of Oil Production for Biodiesel from Oleaginous Yeast Yarrowia lipolytica strain YL-ad9 Using Cassava Waste Pulp as Carbon Source to Meet Indonesia Renewable Energy Need

Mochamad Firmansyah, Muhammad Naufal Hakim, Elisa Frederica Siburian, Ganjar Abdillah Ammar


The fuel consumption numbers of Indonesia are likely to increase to about 450 million kL/year in 2025. Accordingly, the government has launched Indonesia Energy Mix Roadmap 2025 as energy diversification plan for sustainable development. The need for biodiesel in Indonesia is projected to be 10.22 million kL/year. Yarrowiya lilpolytica strain YL-ad9 is a metabolically engineered yeast which capable of producing oils from glucose and is promising as the feedstock of biodiesel. It has great potential to replace vegetable oils from plant which still has lot of limitations mainly come from its slow growth and low oil productivity. It is also can be processed more efficiently compared to microalgae that is still constrained by low biomass concentrations, high dewatering costs, and high water demand. Unlike plant and microalgae, this yeast has several beneficial properties including can grow rapidly, has low water content, has oil yield up to 0.234 g oil/g glucose, and oil productivity can reach 22 kg/m3/day. Moreover, its main carbon source such as glucose can be supplied from cassava waste pulp (CWP) with average production up to 564,415 tons/year in Indonesia. This waste material is very potential because it still contains high starch that can be converted to glucose. By this number of CWP supply, about 0.08 million kL/year biodiesel can be produced. It can be concluded that biodiesel production from CWP using Y. lipolytica strain YL-ad9 is a potential alternative solution to meet the energy needs and support the sustainable development of Indonesia.

Keywords— biodiesel, cassava waste pulp, oil, sustainable development, Yarrowiya lipolytica

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